Takara TB Green Advantage qPCR Premix \200 Rxns

TB Green Advantage qPCR Premix is a convenient 2X master mix containing TB Green dye, full-lengthTaqDNA Polymerase, hot-start antibody, dNTPs, and buffer (including Mg2+). The mix comes with two separate tubes of ROX passive reference dye: ROX Reference Dye LSR contains the optimal concentration of ROX for instruments whose excitation source is a 488 nm laser; and ROX Reference Dye LMP contains the optimal concentration of ROX for instruments whose excitation source is either a lamp or an LED.

Reaction specificity—performance of Takara Bio TB Green Advantage qPCR Premix vs. Competitor Rs mix using a Roche LightCycler.The results for the Takara Bio reagent are shown in Panels A and C, and those for Competitor Rs reagent are shown in Panels B and D. The cycling conditions for our reagent consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 95°C for 5 sec and 60°C for 20 sec. For Competitor Rs reagent, the cycling conditions consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 94°C for 10 sec, 55°C for 5 sec, and 72°C for 10 sec.

Amplification efficiency—performance of TB Green Advantage qPCR Premix vs. Competitor As SYBR mix using an ABI PRISM 7000 Sequence Detection System.The results for the TB Green reagent are shown in Panels A and C, and those for Competitor As reagent are shown in Panels B and D. The cycling conditions for the TB Green reagent consisted of 1 cycle at 95°C for 10 sec, followed by 40 cycles at 95°C for 5 sec and 60°C for 31 sec. For Competitor As reagent, the cycling conditions consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 95°C for 15 sec and 60°C for 1 min.

Reaction specificity—performance of Takara Bios TB Green Advantage qPCR Premix vs. Competitor Is qPCR mix using a Cepheid Smart Cycler.The results for the Takara Bio reagent are shown in Panels A and C, and those for Competitor Is reagent are shown in Panels B and D. The cycling conditions for the TB Green reagent consisted of 1 cycle at 95°C for 2 min, followed by 45 cycles at 95°C for 5 sec and 60°C for 20 sec. For Competitor Is reagent, the cycling conditions consisted of 1 cycle at 95°C for 2 min, followed by 45 cycles at 95°C for 15 sec and 60°C for 30 sec.