Takara NucleoMag DNA/RNA Water \1 x 96 Preps

The NucleoMag DNA/RNA Water kit employs magnetic bead technology to enable efficient isolation of microbial DNA and RNA from diverse air and water samples using manual or automated processing. The kit is compatible with a variety of filters and filtration systems, and can be used to process salt or fresh water samples ranging from turbid to clear. The kit protocol enables efficient removal of inhibitory compounds that might interfere with downstream assays while avoiding the use of reducing agents, and also allows for incorporation of a homogenization step using MN bead tubes for improved sample lysis. Purified DNA and RNA obtained with the kit are suitable for downstream applications such as qPCR and NGS.

Cat. # 744220.1 includes sufficient quantities of reagents and materials for processing 1 x 96 samples.

Overview of NucleoMag DNA/RNA Water protocol for manual processing of 96 samples using NucleoMag SEP.

Efficient detection of microbial DNA from different water and air samples.Various water samples and an air sample were filtered and DNA extracted with the NucleoMag DNA/RNA Water kit was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag DNA/RNA Water kit.

Compatibility with different homogenization devices.Nucleic acids were extracted from spiked or unspiked tapwater samples with the inclusion of a homogenization step on either a Vortex Genie 2 device (using the MN Bead Tube Holder) or a dedicated instrument (Retsch), and resulting yields were measured by qPCR. Both homogenization approaches yielded comparable results for each sample type, indicating the flexibility of the NucleoMag DNA/RNA Water kit in regards to its compatibility with the respective homogenization devices.

Compatibility with different filtration systems.qPCR was performed with nucleic acids isolated from either round filters or a cartridge system using the NucleoMag DNA/RNA Water kit. Comparable CT values were obtained for each prep, demonstrating the versatility of the kit in providing efficient sample processing for each filtration system.

Efficient removal of PCR inhibitors.Serial dilutions were performed on nucleic acids extracted from three different samples (pondwater, seawater, and lakewater) and the dilutions were analyzed by qPCR. The linearity of the inhibition plots indicates the absence of PCR inhibition for each sample.

Automated processing on popular platforms.The NucleoMag DNA/RNA Water kit was used to purify nucleic acids from two different water samples using two different automation platforms, KingFisher (magnetic rods) and epMotion (liquid handling), respectively. qPCR analysis of the purfied nucleic acids indicated that comparable yields were obtained from each sample, indicating the adaptability of the kit to both automation approaches.