Cytiva Pre-Packed Column, 5 mL Vol, 16 mm ID, 2.5 cm L, Polypropylene, 4% Highly Cross-Linked Agarose,

Cytiva pre-packed column, 5 mL vol, 16 mm ID, 2.5 cm L, polypropylene, 4% highly cross-linked agarose, gstrap ff format, 16 x 25 mm bed, <20 mlpm flow rate. gstrap ff columns are pre-packed glutathione sepharose fast flow columns for fast, convenient, one-step purification of glutathione s-transferase (gst) tagged proteins. gst-tagged proteins can be purified directly from pretreated bacterial lysates using gstrap ff.gst-tagged proteins are eluted under mild, non-denaturing conditions using reduced glutathione. the purification process preserves protein antigenicity and function. if desired, cleavage of the protein from gst can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pgex plasmids. gst-tagged proteins can be detected using colorimetric or immunological methods. the resin, glutathione sepharose 4 fast flow, is also available as lab packages and is an excellent choice for scale-up. the columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as äkta design or FPLC system - GHC-F (Additional S&H Or Hazmat Fees May Apply)