Cayman SOAT-1/ACAT-1 Polyclonal Antibody

Sterol O-acyltransferase 1 (SOAT-1), also known as acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT-1), is an enzyme encoded by ACAT1 in humans that catalyzes the intracellular formation of cholesterol esters from cholesterol and long-chain fatty acyl-coenzyme A. It is ubiquitously expressed and localized to the rough endoplasmic reticulum where it preferentially utilizes oleic acid (Item Nos. | ) or palmitic acid (Item No. ) as fatty acid substrates for the synthesis of cholesterol esters, which are stored intracellularly or packaged into chylomicrons or VLDL and secreted into the blood stream. SOAT-1/ACAT-1 protein levels are increased in macrophages under various pathological conditions, including atherosclerosis. SOAT-1/ACAT-1 activity is increased by cholesterol in vitro, and ACAT1 expression is increased by stimulation with the pro-inflammatory cytokines TNF-α or IFN-γ in isolated human and THP-1 monocytes, respectively.^, ACAT1 silencing in human H4 neuroglioma cells overexpressing amyloid-β precursor protein (APP) reduces secretion of soluble amyloid-β (Aβ) and Aβ42 (Item No. ). Genome-wide deletion of Acat1 reduces macrophage infiltration and neutral lipid deposition in atherosclerotic aortic lesions and decreases serum total cholesterol levels, but increases brain cholesterol deposition, in ApoE^-/- mice fed a Western diet. Caymans SOAT-1/ACAT-1 Polyclonal Antibody can be used for immunoflouresence (IF) immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the N-terminus to detect full-length SOAT-1/ACAT-1 at approximately 65 kDa from human, mouse, porcine, and rat samples.

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