Cayman Caspase-3 Monoclonal Antibody (Clone 31A1067)

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation, and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a tetramer to form the active enzyme. The gene for caspase-3 also known as Yama, CPP32, and apopain codes for a 32 kDa protein.^,, Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other caspase-3 substrates include DNA-PK, actin, GAS2, and pro-caspase-6. Caspase-3 is activated by cleavage events at Asp28/Ser²⁹ (between N-terminal pro-domain) and Asp175/Ser¹⁷⁶ (between large and small subunits) to generate a large subunit of 17 kDa and a small subunit of 12 kDa.

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