Cayman Acridine Orange 10-nonyl (bromide)

Acridine orange 10-nonyl is a mitochondrial dye and derivative of acridine orange (Item No. ) that incorporates into the cardiolipin-rich inner mitochondrial membrane. It selectively binds to cardiolipin-containing liposomes over phosphatidylserine- and phosphatidylinositol-containing liposomes in vitro (K_as = 0.002, 7,000, and 7,000 mm, respectively). Acridine orange 10-nonyl binding to mitochondria is abolished by the mitochondrial uncoupler FCCP (Item No. ) in rat cortical astrocytes, neonatal cardiomyocytes, and isolated brain mitochondria, indicating dependence on mitochondrial membrane potential. Acridine orange 10-nonyl displays excitation/emission maxima of 490/510 nm, respectively, and has been used to quantify cardiolipin content and mitochondrial mass in isolated mitochondria and cellular systems.

WARNING This product is not for human or veterinary use.