Cayman RIG-I helicase domain (human, recombinant)

Retinoic acid-inducible gene I (RIG-I), also known as DDX58, is a cytosolic DExD/H-box RNA helicase and an immune sensing receptor encoded by DDX58 in humans. It is composed of a C-terminal repressor domain, a central RNA helicase domain, and two N-terminal caspase recruitment domains (CARDs).^, Following recognition of viral dsRNA by the C-terminal and helicase domains, the helicase domain induces an ATP-dependent conformational change, allowing for interaction of the CARD domains with mitochondrial antiviral-signaling protein (MAVS) and induction of a type I interferon (IFN) response.^,, A single amino acid substitution of phenylalanine for cysteine at position 268 in the RIG-I helicase domain (RIG-I^C268F) leads to enhanced NF-κB activity and expression of IFN-β in basal and poly (I:C)-stimulated cells. Overexpression of RIG-I^C268F in human trabecular meshwork (HTM) cells induces cell death and is positively correlated with glaucoma, a core symptom in the type I interferonopathy atypical Singleton-Merten syndrome.

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