Cayman FSP1 (human, recombinant)

Ferroptosis suppressor protein 1 (FSP1), also known as apoptosis-inducing factor mitochondria-associated 2 (AIFM2), is a flavoprotein and NAD(P)H-dependent oxidoreductase that inhibits ferroptosis in a glutathione peroxidase 4- and glutathione-independent manner.^,, It is encoded by AIFM2 in humans and is composed of a short N-terminal hydrophobic region followed by a flavin adenine dinucleotide-dependent oxidoreductase domain. FSP1 localizes to the plasma membrane in a myristoylation-dependent manner, where it reduces coenzyme Q₁₀ (CoQ₁₀; Item No. ) to CoQ₁₀H₂. Reduced CoQ₁₀ functions as a radical-trapping antioxidant and inhibits lipid peroxidation. AIFM2 expression positively correlates with resistance to glutathione peroxidase 4 (GPX4) inhibitors, including (1S,3R)-RSL3 (Item No. ), mL-210 (Item No. ), and mL-162 (Item No. ) in cancer cell lines. Withdrawal of the ferroptosis inhibitor ferrostatin-1 (Item No. ) reduces tumor growth in an Fsp1/Gpx4 double knockout, but not a Gpx4 single knockout, H460 lung cancer mouse xenograft model, indicating that FSP1 confers resistance to ferroptotic cell death. Caymans FSP1 (human, recombinant) protein can be used for enzyme assay applications.

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