Cayman M-CSF-β (mouse, recombinant)

Macrophage colony-stimulating factor (M-CSF) is a glycoprotein encoded by Csf1 in mice that promotes the differentiation, proliferation, and function of mononuclear phagocytes, including macrophages, osteoclasts, and dendritic cells.¹ Alternative splicing of Csf1 pre-mRNA produces one full-length long isoform (M-CSF-β), an intermediate-length isoform (M-CSF-γ), and a short-length isoform (M-CSF-α) that share sequence homology in the 150-amino acid receptor binding region which is required for the biological activity of M-CSF.^2,3 M-CSF exists as a disulfide-linked homodimer where each monomer contains four α-helices, an anti-parallel β-sheet, and numerous glycosylation sites.¹ M-CSF is constitutively produced by many cell types, including stromal cells, osteoclasts, fibroblasts, and macrophages, and is localized to the cell surface where it can be proteolytically cleaved to yield a secreted form.^4,5 Binding of M-CSF to the M-CSF receptor, which is expressed by monocytes, macrophages, osteoclasts, and dendritic cells, promotes cell differentiation, proliferation, and survival of mononuclear phagocytes and regulates bone resorption by osteoclasts.^6,4 Mice homozygous for Csf1^op, an inactivating mutation, exhibit defects in fertility and neural development and develop osteopetrosis, a condition characterized by increased bone density.⁷ Neutralization of M-CSF with a monoclonal antibody decreases joint swelling and distortion in a mouse model of collagen-induced arthritis.⁸ M-CSF has been used to generate bone marrow- or peripheral blood monocyte-derived macrophages with an anti-inflammatory phenotype in vitro.^9,10 Caymans M-CSF-β (mouse, recombinant) protein can be used for binding and cell-based assay applications. This protein consists of 230 amino acids, has a calculated molecular weight of 26 kDa, and a predicted N-terminus of Lys33 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the observed molecular mass of the protein is approximately 45 kDa due to glycosylation.

WARNING This product is not for human or veterinary use.