Cayman 12-Lipoxygenase (mouse) Polyclonal Antibody

12-Lipoxygenase (12-LO) is one of several LOs that catalyzes the peroxidation of polyunsaturated fatty acids in a position-specific manner. There are four 12-LO subtypes, encoded by distinct genes, that can be categorized according to their tissue expression in mice. Leukocyte-type 12-LO is encoded by Alox15 in mice and shares 74% sequence identity with human ALOX15 but, in contrast to its human ortholog, encodes a 12-lipoxygenating ALOX15 isoform.^,, Leukocyte-type 12-LO exists as a single polypeptide chain composed of an N-terminal regulatory domain and a C-terminal catalytic domain. It catalyzes the oxidation of arachidonic acid (Item Nos. | | ) to 12(S)-HpETE (Item No. ) and, to a lesser extent, 15(S)-HpETE (Item No. ), which are further reduced to 12(S)-HETE (Item No. ) and 15(S)-HETE (Item No. ), respectively.^,, Other substrates of leukocyte-type 12-LO include eicosapentaenoic acid, docosahexaenoic acid, linoleic acid, and γ-linolenic acid, which are oxygenated at various positions in a substrate-dependent manner. Leukocyte-type 12-LO is constitutively expressed at high levels in resident peritoneal macrophages, and its activity is increased by the Th2 cytokines IL-4 and IL-13 in vitro. Alox15^-/- mice are protected from increases in systolic blood pressure in a model of hypertension induced by L-NAME (Item No. ). Caymans 12-Lipoxygenase (leukocyte-type; mouse) Polyclonal Antibody can be used for ELISA and Western blot (WB) applications. The antibody recognizes 12-LO (leukocyte-type) at ~73 kDa from porcine and mouse samples.

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