Cayman SJB2-043

SJB2-043 is an inhibitor of the ubiquitin-specific peptidase 1 (USP1) and USP1-associated factor 1 (UAF1) complex (USP1-UAF1 complex; IC₅₀ = 0.544 µM for its deubiquitinase activity) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) papain-like protease (PL^pro).^1,2 It reduces the levels of USP1, DNA-binding protein inhibitor 1 (ID-1), ID-2, and ID-3 in K562 chronic myelogenous leukemia (CML) cells when used at concentrations ranging from 0.5 to 10 µM, an effect that can be partially blocked by the proteasome inhibitor MG132.¹ SJB2-043 decreases the viability of K562 cells (EC₅₀ = 1.07 µM) and induces apoptosis in K562 cells in a concentration-dependent manner. It also inhibits SARS-CoV-2 PL^pro with IC₅₀ values of 0.56 and 0.091 µM using the fluorogenic substrates Z-LRGG-AMC (Item No. 26641) or ubiquitin-AMC (Ub-AMC), respectively, as PL^pro substrates.²

WARNING This product is not for human or veterinary use.