Cayman Rat Estrogen Receptor Alpha Reporter Assay System

INDIGO’s Rat Estrogen Receptor Alpha Reporter Assay System utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the full-length Rattus norvegicus Estrogen Receptor 1 (nr3a1), a ligand-dependent transcription factor. INDIGOs Reporter Cells include the luciferase reporter gene functionally linked to an ERα-responsive promoter. Thus, quantifying changes in luciferase expression in the test sample-treated reporter cells provides a sensitive surrogate measure of changes in rERα activity. Luciferase gene expression occurs after ligand-bound rERα undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators, and accessory factors required to form a functional transcription complex, culminating in expression of the reporter gene. Unlike some other cell-based assay strategies, the readout from INDIGOs reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo.

The principal application of this assay is in the screening of test samples to quantify any functional bioactivity that they may exert against rat ERa.

rERα Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.[INDIGO Catalog Nos. R00401-32, R00401]

WARNING This product is not for human or veterinary use.