Cayman EGFR Monoclonal Antibody (Clone C225)

Epidermal growth factor receptor (EGFR), also known as HER1 and ErbB1, is a cell surface receptor and member of the EGF family of receptor tyrosine kinases with roles in cell proliferation, differentiation, and survival.^1,2 It is a 170 kDa transmembrane receptor composed of an intracellular tyrosine kinase domain, a transmembrane lipophilic segment, and an extracellular domain that is expressed in epithelial, mesenchymal, and neuronal tissues.^1,2,3 Under unstimulated conditions, EGFR is an auto-inhibited monomer in the plasma membrane.¹ Upon canonical ligand binding, EGFR undergoes homodimerization or heterodimerization with HER2, HER3, or HER4, which induces a conformational change in the cytoplasmic domain that facilitates autophosphorylation and intracellular signaling. EGFR inhibits autophagy under nutrient-rich growth conditions and, conversely, induces autophagy under serum-starved conditions by interacting with the autophagy inhibitor Rubicon to induce its dissociation from Beclin-1. Overexpression of EGFR is found in multiple solid tumors, including renal, breast, ovarian, and head and neck cancer, as well as non-small cell lung cancer (NSCLC).² EGFR^L858R is associated with increased susceptibility to tyrosine kinase inhibition and cell death, while EGFR^T790M is associated with kinase inhibitor resistance in NSCLC.⁴ Inhibition of EGFR reduces angiotensin II-induced cardiac hypertrophy in mice.⁵ Caymans EGFR Chimeric Monoclonal Antibody (Clone C225) was produced recombinantly from the original C225 antibody sequence and can be used for flow cytometry (FC). The C225 antibody was generated by fusing human IgG1 constant domains to the antigen-binding domain of an IgG1 mouse anti-human EGFR monoclonal antibody.^6,7

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