Cayman CD22 Rabbit Monoclonal Antibody (Clone 340)

CD22 is a transmembrane receptor and member of the sialic acid-binding immunoglobulin-type (Ig-type) lectin (SIGLEC) family.^1,2 It is composed of N-terminal extracellular Ig-like variable (IgV) and Ig-like constant 2 (IgC2) domains, a membrane-spanning region, and an intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM) domain. CD22 has two isoforms formed via alternative splicing, CD22β, which is the full-length form and contains six IgC2 domains, and CD22α, which lacks the third and fourth IgC2 domains. It is expressed in the cytosol of premature B cells and on the cell surface of resting and activated B lymphocytes but is not expressed in differentiated B cells.³ CD22 is an inhibitory receptor activated by binding of α2,6-linked sialic acid-containing molecules, such as glycoproteins, which stimulates phosphorylation of tyrosine in the ITIM domain, leading to recruitment of Src homology 2 domain-containing phosphatases (SHPs), including SHP-1, spleen tyrosine kinase (Syk), LYN, and PI3K.^1,2 Knockout of Cd22 decreases IgG1 titers in mice immunized with OVA/alum and expression of human CD22 in Cd22 ^-/- mice rescues this phenotype.⁴ CD22 is overexpressed in cancer cells isolated from patients with hairy cell leukemia.³ Cayman’s CD22 Rabbit Monoclonal Antibody (Clone 340) can be used for ELISA and immunohistochemistry (IHC; paraffin) applications.

WARNING This product is not for human or veterinary use.