Cayman Multi-Parameter Apoptosis Assay Kit; Size- 100 Tests

INDIGO’s Human Constitutive Androstane Receptor, isoform 3 (CAR3) Reporter Assay System utilizes proprietary mammalian cells engineered to provide high-level expression of human CAR3 (NR1I3 isoform 3), a ligand-activated transcription factor. The Reporter Cells utilize a modified version of human CAR3 in which the N-terminal DNA binding domain (DBD) has been replaced with the GAL4-DBD. The human CAR3 ligand binding domain (LBD) is unaltered and fully functional. Reporter cells also incorporate a luciferase cDNA functionally linked to the GAL4-upstream activation sequence (UAS). Thus, quantifying expressed luciferase activity provides a sensitive surrogate measure of changes in CAR3 activity resulting from a direct interaction between a treatment compound and the nuclear receptor. Because this assay system expresses a GAL4-DBD + hCAR3 LBD hybrid receptor, the bio-activity of activators that act through indirect mechanisms (such as those that alter the phosphorylation status of the native N-terminal amino acid sequence of CAR3) may be dampened or go undetected. Human CAR 3 is not constitutively active, rather, it exhibits ligand-dependent activation. The primary application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert on human CAR3. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. CAR3 Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or pre- incubation of reporter cells prior to assay setup. [INDIGO Catalog No. IB00901-32, IB00901, IB00902]

WARNING This product is not for human or veterinary use.