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Empirical’s qPCR Probe MasterMix is a 2X ready-to-use MasterMix for quantitative real time evaluation of DNA using fluorescent probe based detection. Empirical’s qPCR Probe MasterMix contains FlashTaq HotStart DNA Polymerase; a chemically modified Taq DNA polymerase that remains completely inactive at room temperature. The enzyme becomes activated after only 2 minutes at 95°C. Empirical’s qPCR Probe MasterMix includes dNTPs, MgCl 2 , and optimized buffer for fast, efficient qPCR. Empirical’s qPCR Probe MasterMix has been optimized for use with hydrolysis based probes such as TaqMan, but is also suitable with other probe based detection systems.