Sigma-Aldrich Trypsin Inhibitor 1 g

Biological Source

chicken egg white

Quality Level

100

Form

powder

Packaging

pkg of 1 g

Manufacturer/Tradename

Roche

Concentration

1 mg/mL

Technique(S)

cell culture | mammalian: suitable

Optimum pH

3.8-4.5

Solubility

water: soluble

Storage Temp.

2-8°C

Related Categories

Cell Dissociation Reagents

Roche® Life Science Products

General Description

Egg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease).

Application

Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures.Trypsin Inhibitor has been used:

• as a component of lysis buffer to treat Tsc2fl/fl (control) and Tsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting
• to inactivate trypsin post detachment of the stem cells in ventricular zone cell culture
• to neutralize trypsin post dissociation and trypsinization of brain tissues[5]
• termination of tissue disaggregation and for subcultivation procedures

Preparation Note

Working concentration:0
Working solution:Solvent is recommended in distilled water.
Stock solution: 1 mg/mL
Storage conditions (working solution):-15 to -25 °C
Stable for at least 6 months in distilled water or 1 mm HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.

Reconstitution

Both are soluble in water. Recommended stock solution: 1 mg/mL. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months.

Storage And Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

Wgk_Germany

WGK 2

Flash_Point_F

Not applicable

Flash_Point_C

Not applicable