Sigma-Aldrich Nad Approx. 100% Grade I L

Assay

100% (dry weight)

Form

lyophilized

Mol Wt

M_r663.4

Packaging

pkg of 1 g (10127965001)
pkg of 5 g (10127973001)

Manufacturer/Tradename

Roche

Color

off-white

pH

~3.0 (50 mg/mL in water)

Λmax

260 nm

Shipped In

wet ice

Storage Temp.

2-8°C

Related Categories

Roche® Life Science Products

General Description

Nicotinamide adenine dinucleotide (NAD) is a ubiquitouslyfound electron carrier and a cofactor. NAD⁺contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺complexes, the enzyme-cofactor interactions are highly conserved.

Application

NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.NAD has been used inin vitroglycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.[5]

Biochem/Physiol Actions

NAD is also involved in microbial catabolism.[6] β-NAD acts as a substrate for various enzymes in several cellular processes.[7]The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactionsin vivo.[8] It works as an electron acceptor.[9]Electron acceptor

Packaging

Packaged by solid weight.

Quality

Purity:100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A₂₅₀nm - A₂₆₀nm : 0.83 ± 0.02, A₂₈₀nm - A₂₆₀nm : 0.22 ± 0.02
Absence of contaminants:LDH inhibitors are not detectable
Organic solvents:=0.05% (GC)

Specifications

Absorbance coefficients:17.6 x 10³L x mol^-1x cm^-1at 260nm, 5.9 x 10³L x mol^-1x cm^-1at 333nm (CN complex)

Preparation Note

Storage conditions (working solution):10 mg/mL in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.This is the common form of NAD.

Storage Class

13 - Non Combustible Solids

Wgk_Germany

WGK 1

Flash_Point_F

Not applicable

Flash_Point_C

Not applicable