Sigma-Aldrich Singlepath(R) E.Coli Suita

Agency

according to AOAC^®PTM

Quality Level

200

Product Line

Singlepath^®

Shelf Life

24 mo.

Packaging

pack of 25 strips

Manufacturer/Tradename

Singlepath^®

Technique(S)

lateral flow assay: suitable
microbe ID | immunological: suitable

Input

food(s)

Application(S)

food and beverages
pathogen testing

Storage Temp.

2-8°C

Related Categories

Hygiene Monitoring Instruments, Samplers, Tests & Accessories

Pathogen Screening Kits, Instruments & Disposables

General Description

Singlepath^® E. coliis a rapid diagnostic test, designed to detectE. colibacteria in various food and environmental samples. The Singlepath^® E. coliis an immunochromatographic rapid test based on gold-labeled antibodies to detect the presence of a specific antigen, in this case, theE. colibacteria. This lateral flow test is based on the immune flow principle and is a convenient and simple-to-use, one-step device. The test includes a nitrocellulose membrane strip that is coated with antibodies specific toE. coliantigens. When the sample is applied to the test strip, anyE. coliantigens present will bind to the antibodies on the strip, resulting in the development of a visible test line. The test takes approximately 20 minutes to complete, and the results are interpreted based on the presence or absence of the test line. The detection limit can be regarded as less than one colony-forming unit ofE. coliO157 (including H7) in a 25 g food sample, depending on the serogroup. No additional equipment expenditure is needed.

Application

SINGLEPATH^®E. coli O157 is used for pathogen detection in industrial microbiology. This kit is designed for testing food and environmental samples. The test is intended to be used in food-analyzing laboratories for the presumptive qualitative detection ofE. coliO157 (including H7) from a variety of foods. The test has been validated and approved for use in raw ground beef and pasteurized whole milk.

Features And Benefits

• Rapid and reliable method
• High sensitivity and specificity compared to conventional culture methods
• Can be performed without the need for specialized equipment or personnel.

Linkage

Replaces: 1.04141.0002

Analysis Note

Performance test
- Escherichia coli O157:H7 ATCC 35150:
passes test
- E. coli O157:H7 HUSCL8: passes test
- E. coli O55:H7 TB182A: passes testAOAC is a registered trademark of AOAC InternationalSINGLEPATH is a registered trademark of Merck KGaA, Darmstadt, Germany