Sigma-Aldrich Membrane-Filter Rinse Flui

Agency

USP

Quality Level

200

Form

medium granules (dehydrated (DCM))

Packaging

pkg of 500 g

Manufacturer/Tradename

GranuCult^®

Storage Condition

dry media (Tightly closed)

Technique(S)

microbiological culture: suitable

Application(S)

microbiology
pharmaceutical

Storage Temp.

15-25°C

Related Categories

Microbial Culture Media

General Description

Membrane-filter rinse fluid is used to rinse the membrane filter to remove residues of liquid sample materials. It is recommended to use the rinse fluid if the sample contains higher hydrocarbons such as Vaseline, paraffin, etc., or fats. Balanced concentrations of nutrients in the fluid prevent the microorganisms retained by the filter from being exposed to physiological shock, thus enabling them to grow rapidly. The detergent polysorbate 80 ensures the emulsification of carbohydrates and fats without seriously affecting the microorganisms. If the sample contains large quantities of these compounds, additional polysorbate 80 (Tween^®80) (up to 9.0 g/liter) can be added per the USP recommendations before the broth is filtered.

Application

Membrane-filter rinse fluid is used as a rinsing fluid in the membrane filtration procedure.

Features And Benefits

• GranuCult^®offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (color): yellow
pH-value (25 °C): 6.7 - 7.1
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): +
Growth (Streptococcus pyogenes ATCC 12344): +
Growth (Enterococcus faecalis ATCC 11700): +
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): +
Growth (Citrobacter freundii ATCC 8090): +
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): +
Incubation: 18 - 24 hrs.; 35 °C; aerobic streaked on Standard I nutrient agar

Footnote

We offer two media types: the superior granulated GranuCult^®and the cost-efficient powdered NutriSelect^®culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, GermanyNutriSelect is a registered trademark of Merck KGaA, Darmstadt, GermanyTWEEN is a registered trademark of Croda International PLC