Sigma-Aldrich Nad Approx. 98% Lyo. 10 g

Assay

98% (dry weight)

Form

lyophilized

Mol Wt

M_r663.4

Packaging

pkg of 1 g (10127981001)
pkg of 10 g (10621650001)
pkg of 5 g (10127990001)

Manufacturer/Tradename

Roche

Color

off-white

pH

~3.0 (50 mg/mL in water)

Λmax

260 nm

Shipped In

wet ice

Storage Temp.

2-8°C

Related Categories

Roche® Life Science Products

General Description

Nicotinamide adenine dinucleotide (NAD) is a cofactor containing an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. It is ubiquitously found. In NAD⁺complexes, the enzyme-cofactor interactions are highly conserved.

Application

NAD has been used in boiled-blood agar plate as a supplement for the growth ofActinobacillus pleuropneumoniaecolony.

Biochem/Physiol Actions

Nicotinamide adenine dinucleotide (NAD) acts as an electron carrierand significantly participates in enzyme-catalyzed oxido-reduction processesand many genetic processes. NAD cycles between the oxidized (NAD⁺) and reduced (NADH) forms to maintain a redox balance necessary for continued cell growth. This cofactor is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.[5]Electron acceptor

Packaging

Packaged by solid weight.

Quality

Absence of contaminants:LDH inhibitors are not detectable.
Organic solvents:<0.5% (GC)

Preparation Note

Storage conditions (working solution):An aqueous solution in a concentration of 10 mg/mL (pH 2.5 to 3) maintains > 99% of its NAD content after 8 weeks of storage at 2 to 8 °C.
The stability of an aqueous solution in a concentration of 40 mg/mL is not expected to differ from that of a solution in a concentration of 10 mg/mL.

An aqueous solution in a concentration of 50 mg/mL (pH 2.0) maintains 97% of its NAD content after 2 days, 94% after 6 days and 91% after 10 days of storage at 2 to 8 °C and about 83% after 10 days of storage at 33 °C.

An aqueous solution in a concentration of 1.38 mg/mL shows a decrease of NAD content of 4% after 8 weeks of storage at 2 to 8 °C, stored at -15 to -25 °C the stability is expected to be > 6 months. A solution of NAD in triethanolamine buffer (pH 5.0) maintians 90% of its NAD content after
10 days of storage at 2 to 8 °C and 80% after 10 days of storage at 33 °C.

Analysis Note

Absorbance coefficients:17.6 x 10³L x mol^-1x cm^-1at 260 nm, 5.9 x 10³L x mol^-1x cm^-1at 333 nm (CN complex)ß-Nicotinamide adenine dinucleotide hydrate cofactor (NAD) has been used in an experimental protocol, done in order to mimic the citric acid cycle on an carbon electrode, in order to increase biofuel cell energy density.[6]

Other Notes

For life science research only. Not for use in diagnostic procedures.This is the common form of NAD.

Storage Class

13 - Non Combustible Solids

Wgk_Germany

WGK 1

Flash_Point_F

Not applicable

Flash_Point_C

Not applicable