Sigma-Aldrich X-Gal 250mg

Description

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside

Quality Level

100

Assay

99%

Form

solid

Mol Wt

408.63 g/mol

Packaging

pkg of 1 g (10745740001)
pkg of 100 mg (03117073001)
pkg of 100 mg (11680293001)
pkg of 2.5 g (10703729001)
pkg of 250 mg (10651745001)

Manufacturer/Tradename

Roche

Concentration

20 mg/mL

Technique(S)

activity assay: suitable

Color

white

Related Categories

Roche® Life Science Products

General Description

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.

Application

X-Gal has been used for immunohistocytochemistry applications.It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with thelac⁺ genotype.X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with thelac ⁺genotype.

Quality

Chromatographically homogeneous

Physical Form

Crystals, white powder

Preparation Note

Working solution: Stock solution:

• 20 mg/mL in dimethyl formamide (DMF), clear, colorless solution
• A_{590 nm}: 0.01

Storage conditions (working solution):X-gal in solution (i.e.,dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

Wgk_Germany

WGK 2

Flash_Point_F

Not applicable

Flash_Point_C

Not applicable