Sigma-Aldrich Oxford Listeria Selective

Form

solid

Quality Level

200

Composition

Acriflavin, 2.5 mg per vial
Cefotetan, 1 mg per vial
Colistin sulfate, 10 mg per vial
Cycloheximide, 200 mg per vial
Fosfomycin, 5 mg per vial

Packaging

pkg of 10 vials

Technique(S)

microbiological culture: suitable

Application(S)

food and beverages
microbiology

Compatibility

for use with GranuCult^®Oxford Agar (Base)

Storage Temp.

2-8°C

Suitability

selective forListeriaspp.

Related Categories

Supplements for Cell Culture

General Description

OxfordListeriaSelective Supplement is a mixture of four antibiotics and a dye in lyophilized form. It inhibits the growth of accompanying bacteria in the selective cultivation ofListeria monocytogenes.

Application

OxfordListeriaSelective Supplement is an additive for the preparation of OxfordListeriaSelective Agar.

Compatibility

For use with GranuCult^®Oxford Agar (Base) (1.07004)

Linkage

Replaces: 1.07006.0017

Analysis Note

Supplement tested in Oxford Listeria selective agar (base) art. no. 1.07004.
Recovery on test medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
≥ 70 %
- Listeria monocytogenes ATCC 13932 (WDCM 00021): ≥ 70 %
- Listeria monocytogenes ATCC 35152 (WDCM 00109): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
- Enterococcus faecalis ATCC 19433 (WDCM 00009): ≤ 0.01 %
- Escherichia coli ATCC 25922 (WDCM 00013): ≤ 0.01 %
Black zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
+
- Listeria monocytogenes ATCC 13932 (WDCM 00021): +
- Listeria monocytogenes ATCC 35152 (WDCM 00109): +
- Listeria innocua ATCC 33090 (WDCM 00017): +
Growth
- Staphylococcus aureus ATCC 25923 (WDCM 00034):
inhibited
- Candida albicans ATCC 10231 (WDCM 00054): inhibited
Incubation: 48 hrs.; 35 °C; aerobic.
Reference: Tryptic soy agar.GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, GermanyGHS06,GHS08,GHS09H300 - H319 - H341 - H360D - H411P202 - P264 - P273 - P280 - P301 + P310 - P305 + P351 + P338