Sigma-Aldrich Orange Serum Agar Acc. Ifu

Agency

according to IFU

Quality Level

200

Form

medium granules (dehydrated (DCM))

Packaging

pkg of 500 g

Manufacturer/Tradename

GranuCult^®

Storage Condition

protect from light (Keep tightly closed and dry)

Technique(S)

microbiological culture: suitable

Color

beige

pH

5.4-5.6 (37 °C, 42 g/L in H₂O, after autoclaving)

Solubility

42 g/L

Related Categories

Microbial Culture Media

General Description

Orange Serum Agar is developed specifically to isolate and enumerate the microorganisms that can spoil citrus fruit beverages. Due to its low pH, citrus fruit juices are susceptible to spoilage, mainly by molds, yeasts, and acidophilic bacteria such asLactobacillusandLeuconostoc. Orange Serum Agar is recommended by APHA for the examination of fruit beverages. Casein peptone and yeast extract provide essential nitrogenous and carbonaceous nutrients and vitamin B complex. Glucose is added as a carbon and energy source. Orange extract generates an optimal environment for the enrichment of acid-tolerant microorganisms from citrus fruit samples. Dipotassium hydrogen phosphate acts as buffering substance.

Application

Orange Serum agar is suitable for the isolation, cultivation, and enumeration of acid-tolerant spoilage microorganisms in fruit juice and fruit juice concentrates from citrus fruit.

Features And Benefits

• GranuCult^®offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear to opalescent
Appearance (color): yellowish to yellowish-brown
pH-value (25 °C): 5.4 - 5.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)):
Inoculum on reference medium(Lactobacillus plantarum ATCC 14917 (WDCM 00104)):
Inoculum on reference medium (Lactobacillus casei ATCC 393 (WDCM 00100)):
Inoculum on reference medium(Leuconostoc mesenteroides ATCC 9135 (WDCM 00108)):
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Bacillus cereus ATCC 11778 (WDCM 00001)):
Colony count (Lactobacillus plantarum ATCC 14917 (WDCM 00104)):
Colony count (Lactobacillus casei ATCC 393 (WDCM 00100)):
Colony count (Leuconostoc mesenteroides ATCC 9135 (WDCM 00108)):
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≥ 70 %
Recovery on test medium (Lactobacillus plantarum ATCC 14917 (WDCM 00104)): ≥ 70 %
Recovery on test medium (Lactobacillus casei ATCC 393 (WDCM 00100)): ≥ 70 %
Recovery on test medium (Leuconostoc mesenteroides ATCC 9135 (WDCM 00108)): ≥ 70 %
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 70 %
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): good to very good
Incubation: 4 days at 26 ± 2 °C, aerobic
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar resp. SABOURAUD agar

Footnote

We offer two media types: the superior granulated GranuCult^®and the cost-efficient powdered NutriSelect^®culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, GermanyNutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany