Sigma-Aldrich Bactident(Tm) Kovacs Indo

Quality Level

100

Form

liquid (ready-to-use)

Shelf Life

210 days

Feature

ready-to-use

Composition

(n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)

Packaging

pkg of (1 x 30 mL, Plastic bottle)

Technique(S)

microbe ID | specific enzyme detection: suitable

pH

<1 (20 °C in H₂O)

Transition Temp

flash point 36 °C

Density

0.92 g/cm³at 20 °C

Related Categories

Bioprocessing Recombinant Supplements

Microbial Identification Tests

General Description

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

Application

Bactident^™Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification ofE. coli.

Linkage

Replaces: 1.11350.0007

Analysis Note

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.BACTIDENT is a trademark of Merck KGaA, Darmstadt, GermanyGHS02,GHS05,GHS07H226 - H290 - H302 - H314 - H317 - H335 - H336P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338