Sigma-Aldrich Nutrient Agar Acc. Trinkwv

Quality Level

200

Form

medium granules (dehydrated (DCM))

Manufacturer/Tradename

GranuCult^®

Technique(S)

microbiological culture: suitable

pH

7.1-7.5 (37 °C, 40 g/L in H₂O, after autoclaving)

Solubility

40 g/L

Bulk Density

560 kg/M³

Application(S)

food and beverages
microbiology

Storage Temp.

15-25°C

Suitability

heterotrophic bacteria

Related Categories

Microbial Culture Media

Microbial Filtration

Analysis Note

Appearance (clearness): clear
Appearance (color): yellowish brown
pH-value (25 °C): 7.1 - 7.5
Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)):
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 70 %
Incubation: 44 ± 4 hours at 36 ± 1 °C, aerobic
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Footnote

We offer two media types: the superior granulated GranuCult^®and the cost-efficient powdered NutriSelect^®culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, GermanyNutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany