Sigma-Aldrich Dig-11-Utp

Assay

≥85% (HPLC)

Quality Level

100

Form

solution

Mol Wt

(M_r= 1106.7 (DIG-11-UTP-Li4))

Packaging

pkg of 25 μL (11209256910 [10 mm])
pkg of 57 μL (03359247910 [3.5 mm])

Manufacturer/Tradename

Roche

Storage Temp.

−20°C

Related Categories

Nucleic Acid Labeling

Roche® Life Science Products

General Description

Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in thein vitrotranscription reaction for DIG labeling of RNA in a ratio of 35:65%.

Application

Digoxigenin-11-UTP has been used for labeling RNA probes in:

• in situhybridization[5]
• in situreverse transcriptase (RT)-polymerase chain reaction (PCR)[6]
• fluorescencein situhybridization (FISH)[7]

Biochem/Physiol Actions

Linearized template DNA with T7, SP6, or T3 promoter can bein vitrotranscribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.

Quality

Typical analysis:85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.

Formula: C₄₃H₆₁N₄O₂₂P₃Li₄

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

GHS07

Signalword

Warning

Hcodes

H302

Pcodes

P264 - P270 - P301 + P312 + P330 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Wgk_Germany

WGK 1

Flash_Point_F

does not flash

Flash_Point_C

does not flash