Sigma-Aldrich Biotin-16-Ddutp

Quality Level

100

Assay

≥98% (HPLC)

Form

solution

Mol Wt

955.5 (biotin-16-ddUTP-Li₄)

Packaging

pkg of 25 μL (25 nmol; 1mM)

Manufacturer/Tradename

Roche

Concentration

1 mm

Technique(S)

hybridization: suitable

Storage Temp.

−20°C

Related Categories

Roche® Life Science Products

General Description

Biotin-16-ddUTP, tetralithium salt; 1mM

Application

• Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
• DNA polymerase I (Holoenzyme and Klenow fragment)
• T4 DNA polymerase
• Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).

Biotin-16-ddUTP is preferentially used for 3′-end labeling of oligonucleotides with Terminal Transferase, recombinant and labeling DNA.

• The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
• Colony and plaque screening
• In situhybridization

In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example,transcription factors.
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation ofin vitrotranscribed tmRNA during surface plasmon resonance (SPR) technique.It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.

Features And Benefits

Biotin is bound to dideoxyuridine triphosphate via an amide linkage.

Quality

Typical analysis:At least 85% Biotin-16-ddUTP (HPLC, area%).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

Wgk_Germany

nwg

Flash_Point_F

does not flash

Flash_Point_C

does not flash