Sigma-Aldrich PVDF Western Blotting Memb

Material

white PVDF membrane (hydrophobic)

Quality Level

100

Packaging

roll of 1

Manufacturer/Tradename

Roche

Roll W × L

30 cm × 3.00 M

Pore Size

0.2 μm pore size

Shipped In

ambient

Storage Temp.

room temp

Related Categories

Roche® Life Science Products

General Description

PVDF Western Blotting Membranes are hydrophobic, microporous, polyvinylidene difluoride (PVDF) membranes with a pore size of 0.2μm. The PVDF Western Blotting Membranes are ideal for western and DOT blots. The high binding capacity, high mechanical strength, and chemical resistance of the PVDF membrane makes it specifically useful for protein blotting. The combination of strong sample retention and low background binding generates excellent signal-to-noise ratios in colorimetric and chemiluminescent detection. The primary binding mechanism includes electrostatic and hydrophobic nature.
Adsorption: Goat IgG: 294μg/cm²; BSA: 131μg/cm²; insulin: 85μg/cm²

Application

PVDF Western Blotting Membranes has been used in western blot, for transferring proteins for sequencing.
Note: Tested for use with Lumi-Light western blotting substrate or Lumi-Light^Pluswestern blotting substrate.

Quality

Each lot is function tested (DOT blot).

Other Notes

For life science research only. Not for use in diagnostic procedures.