Sigma-Aldrich Trypsin Recomb. Proteomics

Recombinant

expressed inPichia pastoris

Quality Level

100

Assay

95% (gel filtration)

Form

lyophilized

Specific Activity

≥150 units/mg protein

Packaging

pkg of 4 × 100 μg (03708969001)
pkg of 4 × 25 μg (03708985001)

Manufacturer/Tradename

Roche

Storage Temp.

−20°C

Related Categories

Proteases

Roche® Life Science Products

Tissue Dissociation

General Description

This trypsin preparation is generated from the recombinantPichia pastorisstrain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

Specificity

Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

Application

Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.

Preparation Note

Stabilizers:Ca²⁺in mm concentration range
Inhibitors:TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α₂-macroglobulin, α₁-antitrypsin, APMSF, and antipain.
Storage conditions (working solution):-15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!

Reconstitution

Following reconstitution in 10 mm HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

GHS07,GHS08

Signalword

Danger

Hcodes

H315 - H319 - H334 - H335

Pcodes

P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target_Organs

Respiratory system

Storage Class

11 - Combustible Solids

Wgk_Germany

WGK 1

Flash_Point_F

does not flash

Flash_Point_C

does not flash