Sigma-Aldrich Expand Hifi PCR System Dnt

Usage

sufficient for ≤1,000 reactions (04738276001)
sufficient for ≤200 reactions (04738268001)
sufficient for ≤40 reactions (04738250001)

Quality Level

100

Feature

High Fidelity PCR
dNTPs included
hotstart: no

Packaging

pkg of 2,500 U (04738276001 [10x250 U])
pkg of 100 U (04738250001)
pkg of 500 U (04738268001 [2x250 U])

Manufacturer/Tradename

Roche

Technique(S)

PCR: suitable

Input

purified DNA

Storage Temp.

−20°C

Related Categories

PCR Reagents & Kits

Roche® Life Science Products

General Description

Expand High Fidelity PCR System is optimized to efficiently amplify DNA fragments up to 5 kb. The Expand High Fidelity PCR System is composed of a special enzyme mix containing thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. The fidelity of DNA synthesis with Expand High Fidelity PCR System shows a threefold increase compared to Taq DNA Polymerase. T/A cloning is recommended.

Application

The Expand^™High Fidelity PCR System is an enzyme blend delivering superior results with twofold higher yield and threefold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length for:

• PCR
• RT-PCR

For maximum convenience, select the 2x concentrated ready-to-use High Fidelity Master.

Features And Benefits

• Use one enzyme for all applications.Enjoy high yield, fidelity, and flexibility.
• Detect PCR productspreviously undetectable and avoid false negatives.
• Achieve successful resultsfrom small quantities of template DNA.

Packaging

1 kit containing 5 components

Quality

Each lot of Expand High Fidelity is function-tested in PCR using human genomic DNA and specific primers for collagen (1kb amplified product) and the tPA gene (4.8kb amplified product).

Unit Definition

Volume Activity: 3.5U/μL
Each dNTPack contains 10mM additive-free sodium salt nucleotides in a ready-to-use mix.

Other Notes

For life science research only. Not for use in diagnostic procedures.The 5′→3′ polymerase activity of Taq DNA Polymerase and the 3′→5′ exonuclease of the proofreading polymerase combine for improved performance and results; 3′→5′ exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.Expand is a trademark of RocheH412P273 - P501