Sigma-Aldrich Expand Hifi Plus Pcr Syste

Grade

Molecular Biology

Quality Level

100

Form

liquid

Usage

sufficient for ≤125 reactions (04743725001)
sufficient for ≤500 reactions (04743733001)

Feature

High Fidelity PCR
dNTPs included
hotstart: no

Packaging

pkg of 125 U (04743725001)
pkg of 500 U (04743733001 [2x250 U])

Manufacturer/Tradename

Roche

Concentration

50 U/mL (working concentration)

Parameter

72 °C optimum reaction temp.

Technique(S)

PCR: suitable

Related Categories

PCR Reagents & Kits

Roche® Life Science Products

General Description

The Expand High Fidelity^PLUSPCR System is a blend of Taq DNA Polymerase and a thermostable proofreading protein that lacks polymerase activity.
This enzyme blend is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity.
In addition, this enzyme blend also incorporates dUTP and, in combination with Uracil-DNA Glycosylase, can be used to safeguard PCR reactions from cross contamination.
The fidelity of DNA synthesis with the Expand High Fidelity^PLUSPCR System shows a 2-fold increase compared to the fidelity obtained with the Expand High Fidelity PCR System and a 6-fold increase compared to the fidelity obtained with Taq DNA Polymerase.
These characteristics make the Expand High Fidelity^PLUSPCR System blend the product of choice if a larger number of targets need to be amplified at the same time with high yield in combination with improved accuracy. In addition, the product can be used for the efficient labeling of DNA fragments with radioactive or non-radioactive modified nucleotides.

Application

• The Expand^™High Fidelity^PLUSPCR System is designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This convenient dNTPack is the product of choice for the development of a new amplification assay or the optimization of an existing assay. PCR
• RT-PCR
• Primer extension
• Carryover prevention

It has been used in the ultradeep pyrosequencing methods, inverse PCR and to measure the repeat length of CGG sequences.Twofold higher yield and threefold greater fidelity are obtained compared to Taq DNA Polymerase. Use Expand High FidelityPLUS PCR System for:

• PCR up to 5kb
• RT-PCR up to 5kb

Features And Benefits

Expand High FidelityPLUS PCR System is a mixture of Taq DNA polymerase and a DNA polymerase with proofreading activity. The Expand High FidelityPLUS PCR System shows a twofold increase in fidelity compared to the Expand High Fidelity PCR System, and a sixfold increase compared to Taq DNA Polymerase.
By incorporating dUTP, Uracil-DNA Glycosylase can be used to prevent PCR product carryover. Use this kit for efficient labeling of DNA with radioactive or non-radioactive nucleotides. It is optimized for PCR of DNA fragments up to 5kb.
Use 2.5U for a standard 50μl PCR.

• Safeguard against cross-contamination.

This is the choice for large numbers of amplifications.

• Obtain sixfold higher fidelity and higher yields,

compared to Taq DNA Polymerase.

• Efficiently label DNA.

Use this kit to label DNA with radioactive or nonradioactive nucleotides.

• Cost-effective.

Use the convenient solution of premixed PCR grade dNTPs.

Packaging

1 kit containing 5 components

Quality

Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Unit Definition

Volume Activity: 5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.Expand is a trademark of RocheH412P273 - P501