Sigma-Aldrich Ran Translation Inhibitor

Assay

≥96% (HPLC)

Quality Level

100

Form

solid

Potency

9.7 μM K_i

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
desiccated (hygroscopic)
protect from light

Color

orange

Solubility

water: 5 mg/mL
DMSO: 50 mg/mL

Storage Temp.

2-8°C

General Description

A cell permeable indole fused isoquinolinium salt that binds to the expanded repeat (r(GGGGCC)exp (K_d= 9.7 µM) with modest selectivity. This binding is dependent upon the ionic strength and appears to be significantly weaker in the presence of Na+ and K+. Inhibits repeat-associated non-ATG (RAN) translation and foci Formation in r(GGGGCC)66-expressing HEK293 cells and reduces the levels of poly(GP) and poly(GA) proteins (10% at 25 µM; 47% at to 100 µM). Also shown to inhibit RAN translation and foci formation in (GGGGCC)exp-expressing C9ORF72+ iNeurons (~ 2 µM).A cell permeable indole fused isoquinolinium salt that binds to the expanded repeat (r(GGGGCC)exp (K_d= 9.7 µM) with modest selectivity. This binding is dependent upon the ionic strength and appears to be significantly weaker in the presence of Na+ and K+. Inhibits repeat-associated non-ATG (RAN) translation and foci Formation in r(GGGGCC)66-expressing HEK293 cells and reduces the levels of poly(GP) and poly(GA) proteins (10% at 25 µM; 47% at to 100 µM). Also shown to inhibit RAN translation and foci formation in (GGGGCC)exp-expressing C9ORF72+ iNeurons (~ 2 µM).

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Biochem/Physiol Actions

Cell permeable: yesPrimary Target
(r(GGGGCC)expReversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Physical Form

Supplied as HBr salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Su, Z., et Al. 2014.Neuron. 83,1043.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany