IKA Works Habitat Cell Control Tower Package

The HABITAT cell control tower offers an optimized bioreactor-solution for cell culture applications, enabling precise monitoring and control of cell culture processes. It consists of a control unit with comprehensive connectivity options for gas supply, liquid addition, multi-parameter sensors, and temperature regulation, along with a user-friendly tablet interface for seamless bioreactor operation and control.

Control and monitoring

The intuitive software allows easy control and monitoring of the bioprocess and associated parameters via a 10.4-inch tablet. Users can select from batch, fed-batch, and perfusion/continuous operating modes, catering to various cell culture techniques. The innovative Chaotic Mixing function enables efficient and homogeneous mixing when required. The control software provides clear calibration management, real-time data visualization, and complete process documentation, ensuring compliance with FDA CFR Part 11 requirements (optional).

Gas supply

Precise and adjustable gassing is achieved through four individual built-in mass flow controllers for four each separate gas line (N2, O2, air and CO2), enabling flow rates of 0.01 – 2 L / min. tailored to cell growth requirements. By using the integrated and freely customizable cascade control system, each gas flow can be automatically adjusted by the software at any time according to the ambient conditions in order to maintain the target parameters.

Liquid supply

Four integrated Watson-Marlow pumps with adjustable direction and speed offer highest precision and versatility in pumping in and out acid, base, anti-foam agents and feeding solutions for mammalian cell culture media management.

Sensors

HABITAT Bioreactor comes equipped with latest technology sensors to precisely monitor and control critical bioprocess parameters. The following sensors are included in our standard sales vessel packages:

• pH

• DO (dissolved oxygen)

• temperature

• filling level

• foam

Optional sensors: CO2, Conductivity, Turbidity, Viable cell mass, Redox, Off-gas analysis and more.

PID temperature control

The integrated PID control ensures precise temperature regulation at all times, regardless of vessel type (single-wall or double-wall) or fill level. Single-wall glass vessels can be heated with a heating blanket, allowing for accurate temperature settings above room temperature. With the optional use of a cooling finger, it is also possible to achieve temperatures below room temperature. For double-wall vessels, in combination with an IKA thermostat, the temperature range can be freely adjusted between 5-80°C, independent of room conditions or heat generated by the culture itself.

All features at a glance

• compact, space-saving design, dimensions: 223 x 402 x 450 mm (WxDxH)

• latest technology sensors for better insights and more control

• large screen tablet for easy operation (10.4 inch)

• intuitive, easy-to-use control software that provides simple initial setup and intuitive user assistance throughout the process

• four integrated Watson-Marlow pumps

• high precision motor speed control

• cross platform connectivity: USB, PC, RS232, OPC-UA, ethernet, external signal input, external pump, single-use unit, thermostat

• data storage

• four built-in mass flow controllers for gas supply: N2, O2, air, and CO2

• status LED display: direct error display by indicator light

Note: Only a control tower and the separately available vessel package make up a functional unit.

Controller: Cell Growth

Operating mode: timer, continuous and program operation

Display: Tablet PC

Operation: touch screen

Data memory size [GB]: 32

Auto restart after power failure: yes

Ethernet interface: yes

Single use reactor interface: yes

Thermostat interface: yes

Ext. pump interface: yes

Gravimetric feeding: yes

Exponential feeding: yes

Biomass feeding: yes, with Excell Sensor

OPC UA interface: yes

Filter heater: yes

LED light panel: Accessory

Stirring motor: yes

Calibration guide temperature: yes

Calibration guide pH: yes

Calibration guide pO2: yes

pH - temperature compensation: yes

Mode hose filling: yes

Stirrer control mode: off, manual, profile, Cascade-pO2 control, chaotic

Speed range [rpm]: 1 - 2200

Speed deviation >= 100 rpm [%]: ±2

Speed deviation <= 100 rpm [rpm]: ±5

Speed display: TFT

Reversible direction of rotation : yes

Timer: yes

Timer display: TFT

Time setting range [min]: 1 - 120

Heating blanket: yes

Heat output [W]: 250

Heat control: TFT

Temperature control: PID

Working temperature [°C]: 5 - 80

Connection for ext. temperature sensor: PT1000

Temperature display: yes

Temperature measuring range [°C]: 0 - 100

Temperature measurement resolution [K]: 0.1

Temperature control accuracy [°C]: ±0.2

Temperature limit min. [°C]: 0

Temperature limit max. [°C]: 110

pH measurement: yes

pH control mode: off, auto, profile

pH value display: TFT

pH measuring range [pH]: 0 - 14

pH measurement resolution [pH]: 0.01

Accuracy of pH measurement [pH]: ±0.02

Set resolution pH value [pH]: 0.01

pH control accuracy [pH]: ±0.05

Conncetion for pO2 sensor: yes

pO2 control mode: off, manual, auto, profile

Measurement range pO2 min. [%sat]: 0

Measurement range pO2 max. [%sat]: 200

Resolution pO2 measurement [%sat]: 0.01

Accuracy of pO2 measurement [%sat]: ±0.3

pO2 control accuracy [%sat]: 0.3

Conncetion for turbidity sensor: no

Conncetion for conductivity sensor: no

Conncetion for CO2 sensor: no

Exhaust Gas Analyzer: yes, with Blue Vary Sensor

OUR,CER,RQ calculation: yes, with Blue Vary Sensor

RQ feeding: yes, with Blue Vary Sensor

Digital pH sensor: yes, with EasyFerm Bio Arc Sensor

Measuring Range min. digital pH [pH]: 0

Measuring Range max. digital pH [pH]: 14

Accuracy digital pH [pH]: ±0.02

Digital DO sensor: yes, with OxyFerm FDA Arc

Measuring Range min. digital DO [%sat]: 0

Measuring Range max. digital DO [%sat]: 200

Accuracy digital DO [%]: ±2

Optical DO sensor: yes, with VisiFerm RS485-ECS

Measuring Range min. optical DO [%sat]: 0

Measuring Range max. optical DO [%sat]: 300

Accuracy optical DO [%]: ±1

Biomass sensor: yes, with CGQ BioR Sensor

Measuring Range min. Biomass [OD]: 0.5

Measuring Range max. Biomass [OD]: 50

Digital Redox / ORP sensor: yes, with EasyFerm Plus ORP Arc

Measuring Range min. digital ORP [mV]: -1500

Measuring Range max. digital ORP [mV]: 1500

Digital biomass sensor: yes, with Excell Sensor

Measuring Range min. digital biomass [AU]: 0

Measuring Range max. digital biomass [AU]: 6

Accuracy digital biomass [%]: ±1

Viable Cell Density Sensors: yes, with Incyte Sensor

Measuring Range min. cell density [cells/mL]: 5 x 10^5

Measuring Range max. cell density [cells/mL]: 8 x 10^9

Accuracy cell density [%]: ±1

Gas in connection Air [mm]: 6

Gas out connection Air [mm]: 6

Gas in connection O2 [mm]: 6

Gas out connection O2 [mm]: 6

Gas in connection N2 [mm]: 6

Gas out connection N2 [mm]: 6

Gas in connection CO2 [mm]: 6

Gas out connection CO2 [mm]: 6

Aeration mode: continuous & automatic

Display flowrate: l/min, cc/min

Input pressure [mbar]: 200 - 3000

Pressure out min. [mbar]: 200

Pressure out max. [mbar]: 1000

Flowrate Air min. [cc/min]: 10

Flowrate Air max. [cc/min]: 2000

Flowrate O2 min. [cc/min]: 10

Flowrate O2 max. [cc/min]: 2000

Flowrate N2 min. [cc/min]: 10

Flowrate N2 max. [cc/min]: 2000

Flowrate CO2 min. [cc/min]: 10

Flowrate CO2 max. [cc/min]: 2000

Measuring and control accuracy of the gas control units [% fsr]: ±2

Peristaltic pump: yes

Numbers of pump heads: 4

Pump head function: Acid, Base, AntiFoam, Level

Pump head: Watson Marlow 114DV

Number of pump tubes: 4

Number of pump rollers: 4

Flow rate min. [ml/min]: 0.3

Flow rate max. [ml/min]: 270

Flow rate control accuracy [ml/min]: ±0.3

Pump Speed min. [rpm]: 33

Pump Speed max. [rpm]: 400

Pump reversible direction of rotation: yes

Pump speed display: TFT

Inner tube diameter min. [mm]: 0.5

Inner tube diameter max. [mm]: 4.8

Tube outer diamter min. [mm]: 4.1

Tube outer diamter max. [mm]: 8.4

Tube material : Silicone

Tube hardness [Shore A]: 50 - 60

Dimensions (W x H x D) [mm]: 224 x 624 x 405

Weight [kg]: 14

Packing unit [pcs]: 1

Permissible ambient temperature [°C]: 5 - 40

Permissible relative humidity: 80% (up to 31°C), decreasing linearly to max. 50 (@40°C)

Protection class according to DIN EN 60529: IP 20

RS 232 interface: yes

USB interface: USB-B

WiFi Interface: yes

Voltage [V]: 100 - 240

Frequency [Hz]: 50/60

Power input [W]: 300

Power input max. [W]: 1100

Power input standby [W]: 10

Fuse: 2x T5.0A 250V