Takara Retro-X Tet-Off Advanced Inducible Expression System \Each

The Retro-X Tet-Off Advanced Inducible Expression System is a tetracycline-inducible retroviral gene expression system, consisting of retroviral versions of the Tet-Off Advanced regulatory and response vectors. Retroviruses produced from these vectors can be used to generate stable mammalian cell lines that yield high-level, inducible expression of cloned genes under the control of doxycycline. The kit also includes a control vector, a CalPhos Mammalian Transfection Kit, and Tet System Approved FBS.

Retroviral delivery combined with tetracycline-inducible expression.HeLa cells stably transduced with Retro-X Tet-Off Advanced retrovirus were subsequently infected with a Retro-X-Tight-Pur retrovirus engineered for inducible luciferase expression. Infected cells were grown in the absence (–) or presence (+) of Dox for 48 hr and assayed for luciferase activity. RLU = relative light units.

Each Retro-X Tet-Advanced system consists of two retroviral vectors.A regulator vector (e.g., pRetroX-Tet-On Advanced) expresses an optimized tetracycline-controlled transactivator. The response vector (pRetroX-Tight-Pur) contains an improved tetracycline response element (TRE) within thePTightpromoter that controls expression of your gene of interest.