Sigma-Aldrich Trifluoroacetic Acid (25%

Quality Level

200

Form

liquid

Composition

Water, 74.5-75.5% Karl Fischer

Concentration

24.5-25.5% (trifluoroacetic acid)

Density

1.12 g/cm³at 20 °C

Storage Temp.

15-25°C

Smiles String

OC(=O)C(F)(F)F

Inchi

1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)

Inchi Key

DTQVDTLACAAQTR-UHFFFAOYSA-N

Related Categories

Solvents

Application

Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).

Analysis Note

Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %

Pictograms

GHS05

Signalword

Danger

Hcodes

H314 - H412

Pcodes

P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

Storage Class

8B - Non-combustible, corrosive hazardous materials

Wgk_Germany

WGK 2

Flash_Point_F

Not applicable

Flash_Point_C

Not applicable