Quality Bio TRIS HCl, 1M pH 7.5 500ml

Description

Tris HCL is a buffer used in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride provide convenient and highly purified solutions.

Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids.

Applications

Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).

Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.

Storage Conditions: 15°C to 30°C

Shipping Condition: ambient temperature

Sterile Filtered

DNase, RNase and Protease Tested

pH Range: 7.5 ± 0.1

Max Shelf Life: 36 months from date of manufacture

Harmonization Code: 3822.00.5090

ECCN: EAR99