Takara BacPAK Baculovirus GoStix Plus. 20 Tests

BacPAK Baculovirus GoStix Plus provide a rapid, simple, and effective method for instantly quantifying baculovirus in cell culture supernatants. The test involves applying only 20 µl of precious viral supernatant to a GoStix cassette and waiting 10 minutes for the appearance of test and control bands that indicate the presence of baculoviral gp64. The results on the cassette can then be analyzed using ourfree smartphone app, which quantifies titer by comparing the intensities of the test and control bands.

The quantitative output from the GoStix App (referred to as a GoStix Value, or GV) can be used to compare virus amounts between different preparations in a manner similar to, but far easier and quicker than, an ELISA. It also allows for the calculation of actual PFU/mL using a reference virus.

Cat # 631479 contains BacPAK Baculovirus GoStix Plus cassettes, Chase Buffer, and gp64 Control.

Comparison of baculovirus titration methods.A strong correlation was observed between the physical titration methods (GoStix and qPCR) and the functional methods that require infection (ELISA and plaque staining). The time required to obtain results for each method is summarized in the table.

ZsGreen1-expressing baculovirus was generated using the BacPAK Baculovirus Expression System (Cat. # 631402), following the manufacturer’s protocol. Baculovirus supernatant was harvested from several plaques and amplified in fresh Sf21 cells (P1) for four days.

The baculovirus supernatant from a single clone was analyzed using two functional titration methods, the BacPAK Baculovirus Rapid Titer Kit (Cat. # 631406) and the BacPAK p35 ELISA Kit (Single Wash) (Cat. # 631477), as well as two physical titration methods, the BacPAK qPCR Titration Kit (Cat. # 631414) and the BacPAK Baculovirus GoStix Plus (Cat. # 631479).

Infectious units (IFU) and GoStix results from Clone 11 (data not shown) were used as a reference with our online calculator to convert the units from the different methods into IFU for Clone 4 (shown here). The formula used for these conversions is available in the user manual.

Timelines associated with most commonly used methods of baculoviral vector titration.BacPAK GoStix Plus and ELISA methods are significantly faster than traditional methods and DO not require the special equipment of qPCR- and FACS-based methods. FACS = fluorescence-activated cell sorting.

BacPAK GoStix Plus and ELISA baculovirus titration methods can be used at various stages in your baculovirus workflow.BacPAK Baculovirus GoStix Plus (A) can be used for quick titer checks in stages such as clone screening or starting from a stored viral stock for downstream production. BacPAK p35 ELISA Kit (Single Wash) (B) can be used to determine functional titers in steps such as baculovirus titer normalization and expression optimization. You can also use these two products in other stages of your workflow.