Revvity HTRF Human and Mouse Phospho-RNA Pol II (Ser5) Detection Kit, 500 Assay Points

RNA polymerase II (RNA Pol II) is one of the primary enzymes responsible for expressing protein-encoding genes and some small nuclear RNAs. The carboxy-terminal domain (CTD) of RNA Pol II undergoes a cycle of phosphorylation, catalyzed mainly by members of the cyclin-dependent kinase (CDK) family. RNA Pol II CTD phosphorylation allows it to temporally couple transcription with transcription-associated processes (binding specificity of the CTD for particular factors is determined by the site-specific phosphorylation/dephosphorylation events).