Revvity HTRF Human Phospho-HSP27 (Ser78) Detection Kit, 500 Assay Points

Heat shock protein (HSP) 27 (also known as HSPB1) is an ATP-independent, small-sized (approximately 27 kDalton) chaperone molecule. HSP27 is present at basal levels in cells and tissues and is organized as large oligomers up to 1,000 kD. Following phosphorylation (Ser-15, Ser-78, Ser-82, Thr-143), HSP27 reorganizes itself into smaller oligomers, often dimers and tetramers and can interact with other proteins. This simple change in phosphorylation state regulates many of the canonical functions of HSP27. HSP27 is reported to be involved in cell resistance to heat shock and several stress factors, including hypoxia, oxidative stress, infections, and ultraviolet radiation. However, the function of HSP27 is hijacked during disease, and HSP27 helps to promote disease, rather than appropriately regulate cell homeostasis. SO, HSP27 is overexpressed in various cancer cell lines and tumor progression, metastatic potential and treatment resistance have been attributed to the increased HSP27 expression or HSP27 phosphorylation, making HSP27, a therapeutic target of interest.