Revvity AlphaLISA. SureFire Ultra. Human Phospho-PERK (Thr982) Detection Kit, 100 Assay Points

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an ER-resident kinase that is activated in response to ER stress as part of the unfolded protein response (UPR). Upon activation, PERK phosphorylates the eukaryotic initiation factor 2α (eIF2α), leading to a reduction in global protein synthesis, while selectively enhancing the translation of stress response genes. PERK plays a crucial role in maintaining cellular homeostasis and is implicated in various diseases, including neurodegenerative disorders and cancer.

The AlphaLISA SureFire Ultra Human Phospho-PERK (Thr982) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-PERK (Thr982) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening