Revvity AlphaLISA. SureFire Ultra. Human and Mouse Total BAD Detection Kit, 100 Assay Points

Bcl-2-associated death promoter (BAD) is a pro-apoptotic member of the Bcl-2 family of proteins. BAD promotes apoptosis by binding to and neutralizing anti-apoptotic proteins such as Bcl-2 and Bcl-XL, thereby promoting cell death. The function of BAD is regulated through phosphorylation; when phosphorylated, it is sequestered in the cytoplasm, becomes inactive and binds to 14-3-3 proteins, preventing it from inducing apoptosis. Dysregulation of BAD can contribute to cancer progression due to impaired apoptosis.

The AlphaLISA SureFire Ultra Human and Mouse Total BAD Detection Kit is a sandwich immunoassay for the quantitative detection of total BAD in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening