Revvity AlphaLISA. SureFire Ultra. Human Total p16 INK4A Detection Kit, 500 Assay Points

Cyclin-dependent kinase inhibitor 2A (p16 INK4A) is a tumor suppressor protein that inhibits cyclin-dependent kinases 4 and 6 (CDK4/6), thereby regulating the cell cycle by preventing the phosphorylation of retinoblastoma protein (Rb). This action halts cell cycle progression from the G1 to the S phase, thereby inhibiting cellular proliferation. Mutations or deletions in the p16 INK4A gene are associated with several cancers, including melanoma, pancreatic cancer, and head and neck cancers. Elevated levels of p16 INK4A have been also observed in neurodegenerative diseases like Alzheimer’s, where it may contribute to the loss of neuronal function and cognitive decline.

The AlphaLISA SureFire Ultra Human Total p16 INK4A Detection Kit is a sandwich immunoassay for the quantitative detection of total p16 INK4A in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening