Revvity AlphaLISA. SureFire Ultra. Human and Mouse Phospho-MEK2 (Ser217/221) Detection Kit, 500 Assay Points

Mitogen-activated protein kinase kinase 2 (MEK2), also known as MAP2K2, is a dual-specificity protein kinase in the MAPK/ERK signaling pathway. MEK2 phosphorylates and activates ERK1/2, which in turn regulates cell growth, differentiation, and survival. MEK2 is activated by upstream kinases such as RAF, which are often triggered by growth factors and other extracellular signals. Abnormal MEK2 activity is associated with certain cancers, including nearly all cutaneous melanomas. MEK2 is a target for therapeutic intervention in several cancers.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-MEK2 (Ser217/221) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-MEK2 (Ser217/221) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening