Revvity AlphaLISA. SureFire Ultra. Human Total p21 CiP1 Detection Kit, 500 Assay Points

Cyclin-dependent kinase inhibitor 1 (p21 CiP1), also known as CDKN1A, is a potent inhibitor of cyclin-dependent kinases and is involved in cell cycle regulation. p21 inhibits the activity of CDK2 and CDK4 complexes, leading to cell cycle arrest in the G1 phase, and is regulated by the tumor suppressor protein p53 in response to DNA damage.  p21 Cip1 has a dual role in cancer. It can act as a tumor suppressor by inhibiting cell proliferation and promoting DNA repair. However, in some contexts, it can also promote tumorigenesis by inhibiting apoptosis and allowing cancer cells to survive. Increased expression of p21 Cip1 is also associated with cellular senescence, that contributes to aging and age-related diseases.

The AlphaLISA SureFire Ultra Human Total p21 CiP1 Detection Kit is a sandwich immunoassay for the quantitative detection of total p21 CiP1 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening