Revvity AlphaLISA. SureFire Ultra. Human and Mouse Phospho-Rb (Thr821) Detection Kit, 500 Assay Points

Retinoblastoma protein (Rb) is a tumor suppressor protein that regulates the cell cycle by controlling the transition from the G1 to the S phase. Rb achieves this by binding to and inhibiting E2F transcription factors, preventing the transcription of genes required for DNA replication. Loss or mutation of Rb leads to uncontrolled cell proliferation and is associated with various cancers, including retinoblastoma, osteosarcoma, and small cell lung cancer.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-Rb (Thr821) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Rb (Thr821) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening