Revvity AlphaLISA. SureFire Ultra. Human Phospho-eNOS (Thr495) Detection Kit, 500 Assay Points

Endothelial nitric oxide synthase (eNOS) is an enzyme responsible for producing nitric oxide (NO) in blood vessels. NO is a crucial signaling molecule that promotes vasodilation, thereby regulating blood pressure and maintaining vascular homeostasis. eNOS activity is regulated by multiple factors, including calcium levels and phosphorylation status. Dysfunction of eNOS and altered NO production are associated with various cardiovascular diseases, such as hypertension and atherosclerosis.

The AlphaLISA SureFire Ultra Human Phospho-eNOS (Thr495) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-eNOS (Thr495) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening