Revvity Atplite Luminescence Ass

Revvity ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. Discover our ATPlite™ cell viability assay. Our ATPlite and ATPlite 1step luminescence assay systems use patented innovative technologies that measure cell viability, proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity ATPlite is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence cell viability assay is the alternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity Advantages: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Long-lived luminescent signal: half-life (t_1/2) greater than 5 hours, depending on cell type and mediumRapid: results generated in 15 - 25 minutesSimple and reproducible: no separation steps; only two reagent additionsHomogeneous assay: no cell harvesting or centrifugation requiredSensitive: down to 5 cells in 100µL medium (derived from CHO and HL-60 cells in 100µL medium) Wide linear dynamic range: =10⁵ (as derived from CHO and HL-60 cells)