Revvity AlphaLISA SureFire Ultra Human & Mouse Total NF B2 p100/p52 Detection Kit, 50,000 Assay

NF-κ-B2 p100 is a precursor protein of the NF-κ-B family, which plays a key role in regulating immune response, inflammation, and cell survival. p100 can be processed to p52, a transcriptionally active form that enters the nucleus to activate target genes involved in immune response and apoptosis. Dysregulation of NF-κ-B2 is associated with inflammatory diseases, autoimmune diseases, certain lymphomas and multiple myeloma, making it a potential target for therapeutic strategies.

The AlphaLISA SureFire Ultra Human and Mouse Total NFκB p100/p52 Detection Kit is a sandwich immunoassay for the quantitative detection of total NFκB p100/p52 in cellular lysates, using Alpha Technology.

Formats:

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

Cellular kinase assaysReceptor activation studiesScreening