Revvity Igg (Human) Lance Ultra Tr-Fret Detection Kit

Revvity The LANCE® Ultra Human IgG Detection Kit is designed for detection and quantitation of human IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

No-wash steps, no separation stepsTR-FRET technologySensitive detectionHigh reproducibilityFaster time-to-resultsEasy automation96-well, 384-well, and 1536-well formats

Revvity LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity The glycoprotein immunoglobulin g (IgG), a major effector molecule of the humoral immune response in human, accounts for about 75% of the total immunoglobulins in plasma of healthy individuals whereas IgM, IgA, IgD and IgE, each of which has characteristic properties and functions, constitute the remaining 25%. The basic IgG molecule has a four-chain structure, comprising two identical heavy (H) chains and two identical light (L) chains, linked together by interchain disulfide bonds. Four IgG subclasses have been identified showing their most conspicuous differences in the amino acid composition and structure of the hinge region. The present kit recognizes total IgGs Fc fragment. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)